Journal: Neuroscience

Article Title: Deletion of selenoprotein P results in impaired function of parvalbumin interneurons and alterations in fear learning and sensorimotor gating.

doi: 10.1016/j.neuroscience.2012.02.017

Figure Lengend Snippet: Fig. 1. ApoER2 is expressed on PV-interneurons. (A) Images of CA1 region of the hippocampus probed with an ApoER2 antibody in the absence (left panel) and presence (right panel) of an ApoER2 blocking peptide, which left only background staining. (B, C, D) Confocal images showing the expression of ApoER2 (left), PV (middle left), GAD67 (middle right), and co-localization of the markers (right) in the somatosensory cortex (B), hippocampus (C), and inferior colliculus (D) of WT mice. Higher magnification of merged images (far right). Scale bar: 100 m.

Article Snippet: In addition, we also used an ApoER2 blocking peptide (1:25; Santa Cruz, sc-10113 P) to verify the specificity of our anti-ApoER2 antibody.

Techniques: Blocking Assay, Staining, Expressing

Journal: Neuroscience

Article Title: Deletion of selenoprotein P results in impaired function of parvalbumin interneurons and alterations in fear learning and sensorimotor gating.

doi: 10.1016/j.neuroscience.2012.02.017

Figure Lengend Snippet: Fig. 2. ApoER2 is not expressed on calretinin (CR)-interneurons or calbindin (CB)-interneurons. (A, B, C, D) Confocal images showing the expression of ApoER2 (left), CR (A, B) or CB (C, D) (middle left), GAD67 (middle right), and co-localization of the markers (right) in the somatosensory cortex (A, C) and inferior colliculus (B, D) of WT mice. Higher magnification of merged images (far right). Scale bar: 100 m.

Article Snippet: In addition, we also used an ApoER2 blocking peptide (1:25; Santa Cruz, sc-10113 P) to verify the specificity of our anti-ApoER2 antibody.

Techniques: Expressing

Journal: Neuroscience

Article Title: Deletion of selenoprotein P results in impaired function of parvalbumin interneurons and alterations in fear learning and sensorimotor gating.

doi: 10.1016/j.neuroscience.2012.02.017

Figure Lengend Snippet: Fig. 8. Putative model of PV-interneuron function and oxidative stress. Sepp1 binds to ApoER2 and provides selenium for selenopro- tein synthesis. Selenoproteins protect against oxidative stress. Oxida- tive stress impairs NMDA-mediated neurotransmission and, in turn, leads to diminished PV expression. Also included in this model, but beyond the scope of this investigation, are two additional ligands for the ApoER2 receptor, APOE and reelin.

Article Snippet: In addition, we also used an ApoER2 blocking peptide (1:25; Santa Cruz, sc-10113 P) to verify the specificity of our anti-ApoER2 antibody.

Techniques: Expressing